Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: SL disA increases the antigen-presentation capacity of dendritic cells (A) IFN-β levels in BMDC supernatants from media, SL, SL disA , and SL disA +H151 groups. (B, C) BMDCs double-stained with MHC II and CD11c were analyzed by flow cytometry (B), and CD11c + MHCII + cells are shown in the statistical graph (C). Data were expressed as mean ± SEM, n = 3. ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: Cells were subsequently stained with fluorochrome-conjugated antibodies targeting surface markers, including FITC anti-mouse CD11c (clone N418, Elabscience Biotechnology Co., Ltd.) and PE-Cy7 anti-mouse MHC II (clone M5/114, Elabscience Biotechnology Co., Ltd.), and analyzed using a CytoFLEX cytometer (Beckman Coulter, Brea, CA, USA).

Techniques: Immunopeptidomics, Staining, Flow Cytometry

Journal: Journal of Advanced Research

Article Title: The immune regulation and signaling transduction of FAM134B-mediated endoplasmic reticulum-phagy in ferroptosis of dendritic cells after sepsis

doi: 10.1016/j.jare.2025.07.058

Figure Lengend Snippet: FAM134B-mediated ER-phagy downregulates sepsis-induced ferroptosis to protect the immune response of DCs. Two types of mice were adopted the severe CLP. Mice in the control group were defined as a sham operation. A. The percentage of co-stimulatory phenotypes expressed on DCs was measured by flow cytometry. B. DCs from hemophthalmia of CLP mice were cocultured with CD4 + T cells stained with Con A, and then T-cell proliferation induced by DCs was measured by flow cytometry. C. The release of inflammatory cytokines reflecting the degree of DC maturity and secretion was detected by ELISA after CLP. D. The survival rates of WT mice (n = 11) and FAM134B -/- mice (n = 12) were monitored at the indicated time points. E-G. The conditional knockout of FAM134B targeted for splenic DC in mouse (CD11c cre FAM134B fl/fl ) was structured and then underwent CLP procedure. DCs immune functions was measured by Flow Cytometry and survival rates of septic mice were analyzed. Data from three independent experiments were exhibited as the mean ± SD (n = 3 per group). Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001 vs. the WT-Sham group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. the FAM134B -/- -Sham group; & P < 0.05, && P < 0.01, &&& P < 0.001 as the FAM134B -/- -CLP group vs. the WT-CLP group.

Article Snippet: Spleen CD11c + (N418) and CD4 + T cell (L3T4) MicroBeads have been obtained from Miltenyi Biological GmbH (Bergisch Gladbach, Germany).

Techniques: Control, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Knock-Out